pcdna3 ubc9 c93s Search Results


sirna against ntf2  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology sirna against ntf2
Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by <t>siRNA</t> depletion of <t>NTF2</t> in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.
Sirna Against Ntf2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+ubc9+c93s/NTF2+siRNA/pmc03982987-180-52-58
Average 92 stars, based on 1 article reviews
sirna against ntf2 - by Bioz Stars, 2026-10
92/100 stars
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pcdna3 ubc9  (Addgene inc)
91
Addgene inc pcdna3 ubc9
Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by <t>siRNA</t> depletion of <t>NTF2</t> in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.
Pcdna3 Ubc9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+ubc9+c93s/pcDNA3%2FUBC9+(Plasmid+%2320082)/pmc06478910-216-69-85
Average 91 stars, based on 1 article reviews
pcdna3 ubc9 - by Bioz Stars, 2026-10
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pebg bcar1  (Addgene inc)
90
Addgene inc pebg bcar1
Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by <t>siRNA</t> depletion of <t>NTF2</t> in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.
Pebg Bcar1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+ubc9+c93s/pEBG+p130cas+(Plasmid+%2315001)/pmc06478910-216-74-85
Average 90 stars, based on 1 article reviews
pebg bcar1 - by Bioz Stars, 2026-10
90/100 stars
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pebfp n1  (Addgene inc)
96
Addgene inc pebfp n1
Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by <t>siRNA</t> depletion of <t>NTF2</t> in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.
Pebfp N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+ubc9+c93s/pRK5-HA-Ubiquitin-WT+(Plasmid+%2317608)/pmc06478910-216-4-14
Average 96 stars, based on 1 article reviews
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Image Search Results


Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by siRNA depletion of NTF2 in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.

Journal: Molecular Biology of the Cell

Article Title: A pathway linking oxidative stress and the Ran GTPase system in progeria

doi: 10.1091/mbc.E13-07-0430

Figure Lengend Snippet: Ubc9 requires the Ran protein gradient for efficient import. (A) Disruption of the Ran gradient by siRNA depletion of NTF2 in GSN2 cells. HeLa cells (stably transfected with GFP-STV-NLS; Black et al. , 1999 ) were cotransfected with siRNA and Flag-Ubc9 and imaged in three channels for endogenous Ran (purple), Flag-Ubc9 (red), and GFP (green) Scale bars, 20 μm. (B) Histogram (bin size, 0.25) showing Flag-Ubc9 N/C from control siRNA (black) and NTF2 siRNA–transfected (red line) cells. (C) Histogram (bin size, 25) showing nuclear fluorescence intensities of GFP-STV-NLS from control (black) and NTF2 siRNA–transfected (red) cells. (D) xy scatter plot of Ubc9 N/C and Ran N/C in control siRNA (black dots) and NTF2 siRNA (red dots) cells.

Article Snippet: Plasmids encoding the WT and mutant forms of lamin A (pCDNA3-HA-lamin A and pCDNA3-HA-progerin, respectively) were generated as described ( Kelley et al. , 2011 ). pCDNA3-HA-progerin C611S mutant was engineered as described ( Snow et al. , 2013 ). pCMV5-FLAG-Ubc9 and pCMV5-FLAG-Ubc9-C93S were generously provided by David Wotton, University of Virginia. siRNA against NTF2 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Disruption, Stable Transfection, Transfection, Control, Fluorescence

Blocking pre–lamin A processing with LPV disrupts the Ran gradient and induces ROS. (A) Immunoblotting for pre–lamin A, lamin A, and tubulin (loading control) in control and lamin A siRNA–transfected normal (8469) human fibroblasts treated with and without LPV. (B) Bar graph depicting the mean DCF fluorescence in control and lamin A siRNA–transfected cells with and without LPV treatment. (C) IF detection of pre–lamin A (red) and Ran (green) in control and lamin A siRNA–transfected cells with or without LPV treatment. Scale bars, 20 μm. (D) Bar graph depicting mean Ran N/C in control and lamin A siRNA–transfected cells with and without LPV treatment. Statistical significance as measured by ANOVA with a Tukey posttest: *** p < 0.001.

Journal: Molecular Biology of the Cell

Article Title: A pathway linking oxidative stress and the Ran GTPase system in progeria

doi: 10.1091/mbc.E13-07-0430

Figure Lengend Snippet: Blocking pre–lamin A processing with LPV disrupts the Ran gradient and induces ROS. (A) Immunoblotting for pre–lamin A, lamin A, and tubulin (loading control) in control and lamin A siRNA–transfected normal (8469) human fibroblasts treated with and without LPV. (B) Bar graph depicting the mean DCF fluorescence in control and lamin A siRNA–transfected cells with and without LPV treatment. (C) IF detection of pre–lamin A (red) and Ran (green) in control and lamin A siRNA–transfected cells with or without LPV treatment. Scale bars, 20 μm. (D) Bar graph depicting mean Ran N/C in control and lamin A siRNA–transfected cells with and without LPV treatment. Statistical significance as measured by ANOVA with a Tukey posttest: *** p < 0.001.

Article Snippet: Plasmids encoding the WT and mutant forms of lamin A (pCDNA3-HA-lamin A and pCDNA3-HA-progerin, respectively) were generated as described ( Kelley et al. , 2011 ). pCDNA3-HA-progerin C611S mutant was engineered as described ( Snow et al. , 2013 ). pCMV5-FLAG-Ubc9 and pCMV5-FLAG-Ubc9-C93S were generously provided by David Wotton, University of Virginia. siRNA against NTF2 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Blocking Assay, Western Blot, Control, Transfection, Fluorescence

Disruption of the Ran protein gradient is sufficient to induce ROS. (A) Disruption of the Ran gradient by siRNA depletion of NTF2 in 8469 cells (5 d), followed by IF microscopy detection of Ran distribution. Scale bars, 20 μm. (B) ROS detection in 8469 fibroblasts treated with control and NTF2 siRNA, showing an increase in levels upon NTF2 knockdown. Scale bars, 100 μm. (C) Histogram depicting an increase in mean DCF fluorescence intensity upon NTF2 knockdown (red lines) in comparison to control siRNA–treated cells (black bars). (D) ROS detection in 8469 fibroblasts treated with control and Ran siRNA. (E) Histogram depicting an increase in mean DCF fluorescence intensity upon Ran knockdown (red lines) in comparison to control (black bars). Bin sizes, 10.

Journal: Molecular Biology of the Cell

Article Title: A pathway linking oxidative stress and the Ran GTPase system in progeria

doi: 10.1091/mbc.E13-07-0430

Figure Lengend Snippet: Disruption of the Ran protein gradient is sufficient to induce ROS. (A) Disruption of the Ran gradient by siRNA depletion of NTF2 in 8469 cells (5 d), followed by IF microscopy detection of Ran distribution. Scale bars, 20 μm. (B) ROS detection in 8469 fibroblasts treated with control and NTF2 siRNA, showing an increase in levels upon NTF2 knockdown. Scale bars, 100 μm. (C) Histogram depicting an increase in mean DCF fluorescence intensity upon NTF2 knockdown (red lines) in comparison to control siRNA–treated cells (black bars). (D) ROS detection in 8469 fibroblasts treated with control and Ran siRNA. (E) Histogram depicting an increase in mean DCF fluorescence intensity upon Ran knockdown (red lines) in comparison to control (black bars). Bin sizes, 10.

Article Snippet: Plasmids encoding the WT and mutant forms of lamin A (pCDNA3-HA-lamin A and pCDNA3-HA-progerin, respectively) were generated as described ( Kelley et al. , 2011 ). pCDNA3-HA-progerin C611S mutant was engineered as described ( Snow et al. , 2013 ). pCMV5-FLAG-Ubc9 and pCMV5-FLAG-Ubc9-C93S were generously provided by David Wotton, University of Virginia. siRNA against NTF2 was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Disruption, Microscopy, Control, Knockdown, Fluorescence, Comparison